Friday, April 18, 2014
YOLO COUNTY NEWS
99 CENTS

Cell response to electrical fields may open doors to treatments

By
From page A10 | April 07, 2013 | Leave Comment

Min Zhao is a professor of dermatology and ophthalmology and a researcher at UC Davis' stem cell center, the Institute for Regenerative Cures. Courtesy photo

Like tiny, crawling compass needles, whole living cells and cell fragments orient and move in response to electric fields — but in opposite directions, UC Davis scientists have found.

Their results, published April 8 in the journal Current Biology, ultimately could lead to new ways to heal wounds and deliver stem cell therapies.

When cells crawl into wounded flesh to heal it, they follow an electric field. In healthy tissue there’s a flux of charged particles between layers. Damage to tissue sets up a “short circuit,” changing the flux direction and creating an electrical field that leads cells into the wound. But exactly how and why does this happen? That’s unclear.

“We know that cells can respond to a weak electrical field, but we don’t know how they sense it,” said Min Zhao, professor of dermatology and ophthalmology and a researcher at UC Davis’ stem cell center, the Institute for Regenerative Cures. “If we can understand the process better, we can make wound healing and tissue regeneration more effective.”

The researchers worked with cells that form fish scales, called keratocytes. These fish cells are commonly used to study cell motion, and they also readily shed cell fragments, wrapped in a cell membrane but lacking a nucleus, major organelles, DNA or much else in the way of other structures.

In a surprise discovery, whole cells and cell fragments moved in opposite directions in the same electric field, said Alex Mogilner, professor of mathematics and of neurobiology, physiology and behavior at UCD and co-senior author of the paper.

It’s the first time that such basic cell fragments have been shown to orient and move in an electric field, Mogilner said. That allowed the researchers to discover that the cells and cell fragments are oriented by a “tug of war” between two competing processes.

Think of a cell as a blob of fluid and protein gel wrapped in a membrane. Cells crawl along surfaces by sliding and ratcheting protein fibers inside the cell past each other, advancing the leading edge of the cell while withdrawing the trailing edge.

Assistant project scientist Yaohui Sun found that when whole cells were exposed to an electric field, actin protein fibers collected and grew on the side of the cell facing the negative electrode (cathode), while a mix of contracting actin and myosin fibers formed toward the positive electrode (anode). Both actin alone, and actin with myosin, can create motors that drive the cell forward.

The polarizing effect set up a tug-of-war between the two mechanisms. In whole cells, the actin mechanism won, and the cell crawled toward the cathode. But in cell fragments, the actin/myosin motor came out on top, got the rear of the cell oriented toward the cathode, and the cell fragment crawled in the opposite direction.

The results show that there are at least two distinct pathways through which cells respond to electric fields, Mogilner said. At least one of the pathways — leading to organized actin/myosin fibers — can work without a cell nucleus or any of the other organelles found in cells, beyond the cell membrane and proteins that make up the cytoskeleton.

Upstream of those two pathways is some kind of sensor that detects the electric field. In a separate paper to be published in the same journal issue, Mogilner and Stanford University researchers Greg Allen and Julie Theriot narrow down the possible mechanisms. The most likely explanation, they conclude, is that the electric field causes certain electrically charged proteins in the cell membrane to concentrate at the membrane edge, triggering a response.

The team also included Hao Do, Jing Gao and Ren Zhao, all at the Institute for Regenerative Cures and the UCD departments of Ophthalmology and Dermatology. Sun is co-advised by Mogilner and Zhao; Gao is now working at Yunnan Normal University, Kunming, China, and Ren Zhao is at the Third Military Medical University, Chongqing, China.

The work was funded by the National Institutes of Health, the California Institute for Regenerative Medicine and the National Science Foundation.

— UC Davis News Service

LEAVE A COMMENT

Discussion | No comments

The Davis Enterprise does not necessarily condone the comments here, nor does it review every post. Read our full policy

  • Recent Posts

  • Enter your email address to subscribe to this newspaper and receive notifications of new articles by email.

  • .

    News

    Going green at church, school, everywhere

    By Special to The Enterprise | From Page: A1 | Gallery

     
    Former caretaker convicted of murder, elder abuse

    By Lauren Keene | From Page: A1, 4 Comments | Gallery

    Old friend helps Brad and others find kidneys

    By Dave Jones | From Page: A1 | Gallery

     
    Chuck Rairdan joins school board race

    By Jeff Hudson | From Page: A1, 1 Comment

     
    Ukraine insurgents reject call to quit buildings

    By The Associated Press | From Page: A2, 2 Comments

     
    For the record

    By Enterprise staff | From Page: A2

    UCD to host Global Health Day event

    By Cory Golden | From Page: A2

     
    Need a new best friend?

    By Enterprise staff | From Page: A3 | Gallery

     
    ‘Hitchhiking’ dog looking for new home

    By Lauren Keene | From Page: A3 | Gallery

     
    Online K-12 school holds info night

    By Enterprise staff | From Page: A4

    Volkssporting Club plans North Davis walks

    By Enterprise staff | From Page: A4

     
    Schwenger lawn signs available

    By Anne Ternus-Bellamy | From Page: A4

    Volunteers needed for Grad Night

    By Enterprise staff | From Page: A4

     
    Davis grad makes rain collection a business

    By Jason McAlister | From Page: A4 | Gallery

    A few spots left on history tour

    By Enterprise staff | From Page: A4

     
    Chipotle fundraiser boosts Emerson tech upgrade

    By Enterprise staff | From Page: A4

    Event to provide nature scholarship

    By Enterprise staff | From Page: A4

     
    Students have new options on leasing front

    By Enterprise staff | From Page: A5 | Gallery

    Groups join for a day of service

    By Special to The Enterprise | From Page: A7

     
    NAMI backers walk in Sacramento

    By Special to The Enterprise | From Page: A8

     
    Food for the hungry

    By Enterprise staff | From Page: A10

    .

    Forum

    Dad makes mom look bad

    By Special to The Enterprise | From Page: B5

     
    Early help is a great investment

    By Letters to the Editor | From Page: A6

    More tax money? Answer the question

    By Rich Rifkin | From Page: A6, 2 Comments

     
    UCD IS responsible for students

    By Letters to the Editor | From Page: A6, 2 Comments

     
    Tom Meyer cartoon

    By Debbie Davis | From Page: A6

    In search of great ideas

    By Letters to the Editor | From Page: A6, 1 Comment

     
    Please keep the nursery open

    By Letters to the Editor | From Page: A6

    .

    Sports

    Sharks double up Kings in Game 1

    By The Associated Press | From Page: B1

     
    Aggies lose a slugfest in opener at Riverside

    By Enterprise staff | From Page: B1

    Fox coming to UCD; Riffle heads to Florida

    By Bruce Gallaudet | From Page: B1

     
    DHS’ Golston goes full-bore on the diamond

    By Thomas Oide | From Page: B1 | Gallery

    Devils show more life in loss to Mitty

    By Enterprise staff | From Page: B1

     
    DYSA roundup: Intensity has big week; 10U games dominate schedule

    By Enterprise staff | From Page: B2 | Gallery

    Sports briefs: Aggies set the academic bar high

    By Enterprise staff | From Page: B8 | Gallery

     
    Pro baseball roundup: Susac sends Sacramento to a rare loss

    By The Associated Press | From Page: B8

    .

    Features

    .

    Arts

    ‘Transcendence’: A whole new level of tedium

    By Derrick Bang | From Page: A11 | Gallery

     
    ‘The Bloom’ paves way for Whole Earth Festival

    By Enterprise staff | From Page: A11

    DHS tribute to Tony Fields slated for April 25-26

    By Enterprise staff | From Page: A11

     
    UCD, city team up for Music on the Green

    By Enterprise staff | From Page: A11

    .

    Business

    Ford turns its Focus to domestic market

    By Ali Arsham | From Page: B3 | Gallery

     
    .

    Obituaries

    .

    Comics

    Comics: Friday, April 18, 2014

    By Creator | From Page: A9

     
    .

    Real Estate Review

    Featured Listing

    By Zack Snow | From Page: RER1

    Professional Services Directory

    By Zack Snow | From Page: RER2

    Lyon Real Estate

    By Zack Snow | From Page: RER3

    Acacia at Huntington Square

    By Zack Snow | From Page: RER4

    Jamie Madison

    By Zack Snow | From Page: RER4

    Yolo FCU

    By Zack Snow | From Page: RER4

    Travis Credit Union

    By Zack Snow | From Page: RER5

    Kim Eichorn

    By Zack Snow | From Page: RER6

    Suzanne Kimmel

    By Zack Snow | From Page: RER6

    Lynne Wegner

    By Zack Snow | From Page: RER7

    Kim Merrel Lamb

    By Zack Snow | From Page: RER7

    Patricia Echevarria

    By Zack Snow | From Page: RER8

    Chris Snow

    By Zack Snow | From Page: RER8

    Sheryl Patterson

    By Zack Snow | From Page: RER9

    Don Guthrie

    By Zack Snow | From Page: RER9

    Andrew Dowling

    By Zack Snow | From Page: RER9

    Coldwell Banker

    By Zack Snow | From Page: RER10

    Coldwell Banker

    By Zack Snow | From Page: RER11

    Heather Barnes

    By Zack Snow | From Page: RER12

    Julie Partain & Dick Partain

    By Zack Snow | From Page: RER12

    Malek Baroody

    By Zack Snow | From Page: RER13

    Karen Waggoner

    By Zack Snow | From Page: RER14

    Willowbank Park

    By Zack Snow | From Page: RER14

    Julie Leonard

    By Zack Snow | From Page: RER15

    Tim Harrison

    By Zack Snow | From Page: RER15

    Team Traverso

    By Zack Snow | From Page: RER15

    Tracy Harris

    By Zack Snow | From Page: RER16

    Lori Prizmich

    By Zack Snow | From Page: RER16

    Joe Kaplan

    By Zack Snow | From Page: RER17

    Raul Zamora

    By Zack Snow | From Page: RER17

    Coldwell Banker

    By Zack Snow | From Page: RER18

    Open House Map

    By Zack Snow | From Page: RER19

    F1rst Street Real Estate

    By Zack Snow | From Page: RER20